RUB » Department of Biophysics » X-ray structure analysis of proteins


See also ORCID iD

Vinklárek IS, Bornemann TLV, Lokstein H, Hofmann E, Alster J, Pšenčík J: Temperature Dependence of Chlorophyll Triplet Quenching in Two Photosynthetic Light-Harvesting Complexes from Higher Plants and Dinoflagellates. J Phys Chem B 2018, 122:8834–8845.
Ledermann B, Schwan M, Sommerkamp JA, Hofmann E, Béjà O, Frankenberg‐Dinkel N: Evolution and molecular mechanism of four-electron reducing ferredoxin-dependent bilin reductases from oceanic phages. The FEBS Journal 2018, 285:339–356.
Agostini A, Niklas J, Schulte T, Di Valentin M, Bortolus M, Hofmann E, Lubitz W, Carbonera D: Changing the site energy of per-614 in the Peridinin-chlorophyll a-protein does not alter its capability of chlorophyll triplet quenching. Biochimica et Biophysica Acta (BBA) - Bioenergetics 2018, 1859:612–618.
Winkler M, Senger M, Duan J, Esselborn J, Wittkamp F, Hofmann E, Apfel U-P, Stripp ST, Happe T: Accumulating the hydride state in the catalytic cycle of [FeFe]-hydrogenases. Nature Communications 2017, 8:16115.
Kertess L, Wittkamp F, Sommer C, Esselborn J, Rüdiger O, Reijerse EJ, Hofmann E, Lubitz W, Winkler M, Happe T, et al.: Chalcogenide substitution in the [2Fe] cluster of [FeFe]-hydrogenases conserves high enzymatic activity. Dalton Trans 2017, 46:16947–16958.
Gasper R, Schwach J, Hartmann J, Holtkamp A, Wiethaus J, Riedel N, Hofmann E, Frankenberg-Dinkel N: Distinct Features of Cyanophage-encoded T-type Phycobiliprotein Lyase ΦCpeT THE ROLE OF AUXILIARY METABOLIC GENES. J Biol Chem 2017, 292:3089–3098.
Yip KT, Zhong XY, Seibel N, Pütz S, Autzen J, Gasper R, Hofmann E, Scherkenbeck J, Stoll R: Small Molecules Antagonise the MIA-Fibronectin Interaction in Malignant Melanoma. Scientific Reports 2016, 6:25119.
Noth J, Esselborn J, Güldenhaupt J, Brünje A, Sawyer A, Apfel U-P, Gerwert K, Hofmann E, Winkler M, Happe T: [FeFe]-Hydrogenase with Chalcogenide Substitutions at the H-Cluster Maintains Full H2 Evolution Activity. Angew Chem 2016, 128:8536–8540.
Kvíčalová Z, Alster J, Hofmann E, Khoroshyy P, Litvín R, Bína D, Polívka T, Pšenčík J: Triplet–triplet energy transfer from chlorophylls to carotenoids in two antenna complexes from dinoflagellate Amphidinium carterae. Biochimica et Biophysica Acta (BBA) - Bioenergetics 2016, 1857:341–349.
Gasper R, Effenberger I, Kolesinski P, Terlecka B, Hofmann E, Schaller A: Dirigent Protein Mode of Action Revealed by the Crystal Structure of AtDIR6. Plant Physiol 2016, 172:2165–2175.
Esselborn J, Muraki N, Klein K, Engelbrecht V, Metzler-Nolte N, Apfel U-P, Hofmann E, Kurisu G, Happe T: A structural view of synthetic cofactor integration into [FeFe]-hydrogenases. Chem Sci 2016, 7:959–968.
Di Valentin M, Dal Farra MG, Galazzo L, Albertini M, Schulte T, Hofmann E, Carbonera D: Distance measurements in peridinin-chlorophyll a-protein by light-induced PELDOR spectroscopy. Analysis of triplet state localization. Biochimica et Biophysica Acta (BBA) - Bioenergetics 2016, 1857:1909–1916.
Liauw P, Kannchen D, Gasper R, Dyczmons-Nowaczyk N, Nowaczyk MM, Hofmann E: Cloning, expression, crystallization and preliminary X-ray studies of a superfolder GFP fusion of cyanobacterial Psb32. Acta Crystallographica Section F Structural Biology Communications 2015, 71:409–413.
Dünschede B, Träger C, Schröder CV, Ziehe D, Walter B, Funke S, Hofmann E, Schünemann D: Chloroplast SRP54 Was Recruited for Posttranslational Protein Transport via Complex Formation with Chloroplast SRP43 during Land Plant Evolution. J Biol Chem 2015, 290:13104–13114.
Polivka T, Hofmann E: Structure-Function Relationship in Peridinin-Chlorophyll Proteins. In The Structural Basis of Biological Energy Generation. . Springer; 2014:39–58.
Overkamp KE, Gasper R, Kock K, Herrmann C, Hofmann E, Frankenberg-Dinkel N: Insights into the Biosynthesis and Assembly of Cryptophycean Phycobiliproteins. J Biol Chem 2014, 289:26691–26707.
Šlouf V, Fuciman M, Johanning S, Hofmann E, Frank HA, Polívka T: Low-temperature time-resolved spectroscopic study of the major light-harvesting complex of Amphidinium carterae. Photosynth Res 2013, 117:257–265.
Olejnik M, Krajnik B, Kowalska D, Twardowska M, Czechowski N, Hofmann E, Mackowski S: Imaging of fluorescence enhancement in photosynthetic complexes coupled to silver nanowires. Applied Physics Letters 2013, 102:083703-083703–5.
Makbul C, Constantinescu Aruxandei D, Hofmann E, Schwarz D, Wolf E, Herrmann C: Structural and Thermodynamic Characterization of Nore1-SARAH: A Small, Helical Module Important in Signal Transduction Networks. Biochemistry 2013, 52:1045–1054.
Krajnik B, Gajda-Rączka M, Piątkowski D, Nyga P, Jankiewicz B, Hofmann E, Mackowski S: Silica nanoparticles as a tool for fluorescence collection efficiency enhancement. Nanoscale Research Letters 2013, 8:146.
Krajnik B, Czechowski N, Piatkowski D, Mackowski S, Hofmann E, Pichler S, Heiss W: Influence of Plasmon Excitations in Au Nanoparticles upon Fluorescence and Photostability of Photosynthetic Complexes. Optics and Photonics Journal 2013, 03:1–7.
Kowalska D, Krajnik B, Olejnik M, Twardowska M, Czechowski N, Hofmann E, Mackowski S: Metal-Enhanced Fluorescence of Chlorophylls in Light-Harvesting Complexes Coupled to Silver Nanowires. The Scientific World Journal 2013, 2013.
Träger C, Rosenblad MA, Ziehe D, Garcia-Petit C, Schrader L, Kock K, Vera Richter C, Klinkert B, Narberhaus F, Herrmann C, et al.: Evolution from the Prokaryotic to the Higher Plant Chloroplast Signal Recognition Particle: The Signal Recognition Particle RNA is Conserved in Plastids of a Wide Range of Photosynthetic Organisms. The Plant Cell 2012, 24:4819–4836.
Syberg F, Suveyzdis Y, Kötting C, Gerwert K, Hofmann E: Time-resolved Fourier Transform Infrared Spectroscopy of the Nucleotide-binding Domain from the ATP-binding Cassette Transporter MsbA ATP Hydrolysis is the Rate-limiting Step in the Catalytic Cycle. J Biol Chem 2012, 287:23923–23931.
Müller A, Schlicker C, Fehringer M, Masepohl B, Hofmann E: Expression, purification, crystallization and preliminary X-ray analysis of the DNA-binding domain of Rhodobacter capsulatus MopB. Acta Crystallographica Section F: Structural Biology and Crystallization Communications 2011, 67:377–379.
Busch AWU, Reijerse EJ, Lubitz W, Hofmann E, Frankenberg‑Dinkel N: Radical mechanism of cyanophage phycoerythrobilin synthase (PebS). Biochem J 2011, 433:469–476.
Busch A, Reijerse E, Lubitz W, Frankenberg-Dinkel N, Hofmann E: Structural and mechanistic insights into the ferredoxin-mediated two electron reduction of bilins. Biochem J 2011, 433:469–476.
Wolf S, Freier E, Potschies M, Hofmann E, Gerwert K: Directional proton transfer in membrane proteins achieved through protonated protein-bound water molecules: a proton diode. Angew Chem Int Ed Engl 2010, 49:6889–6893.
Schulte T, Johanning S, Hofmann E: Structure and function of native and refolded peridinin-chlorophyll-proteins from dinoflagellates. Eur J Cell Biol 2010, 89:990–997.
Schulte T, Hiller RG, Hofmann E: X-ray structures of the peridinin-chlorophyll-protein reconstituted with different chlorophylls. FEBS Lett 2010, 584:973–978.
Krajnik B, Schulte T, Piatkowski D, Czechowski N, Hofmann E, Mackowski S: SIL-based confocal fluorescence microscope for investigating individual nanostructures. Cent Eur J Phys 2010,
Schulte T, Sharples FP, Hiller RG, Hofmann E: X-ray structure of the high-salt form of the peridinin-chlorophyll a-protein from the dinoflagellate Amphidinium carterae: modulation of the spectral properties of pigments by the protein environment. Biochemistry 2009, 48:4466–4475.
Schulte T, Niedzwiedzki DM, Birge RR, Hiller RG, Polívka T, Hofmann E, Frank HA: Identification of a single peridinin sensing Chl-a excitation in reconstituted PCP by crystallography and spectroscopy. Proc Natl Acad Sci USA 2009, 106:20764–20769.
Bujak L, Piatkowski D, Mackowski S, Wormke S, Jung C, Brauchle C, Agarwal A, Kotov N, Schulte T, Hofmann E, et al.: Plasmon Enhancement of Fluorescence in Single Light-Harvesting Complexes from Amphidinium carterae. Acta Phys Pol A 2009, 116:S22–S25.
Schaller F, Zerbe P, Reinbothe S, Reinbothe C, Hofmann E, Pollmann S: The allene oxide cyclase family of Arabidopsis thaliana: localization and cyclization. FEBS J 2008, 275:2428–2441.
Hofmann E, Pollmann S: Molecular mechanism of enzymatic allene oxide cyclization in plants. Plant Physiol Biochem 2008, 46:302–308.
Dammeyer T, Hofmann E, Frankenberg-Dinkel N: Phycoerythrobilin synthase (PebS) of a marine virus. Crystal structures of the biliverdin complex and the substrate-free form. J Biol Chem 2008, 283:27547–27554.
Brotosudarmo THP, Mackowski S, Hofmann E, Hiller RG, Bräuchle C, Scheer H: Relative binding affinities of chlorophylls in peridinin-chlorophyll-protein reconstituted with heterochlorophyllous mixtures. Photosyn Res 2008, 95:247–252.
Wörmke S, Mackowski S, Brotosudarmo THP, Bräuchle C, Garcia A, Braun P, Scheer H, Hofmann E: Detection of single biomolecule fluorescence excited through energy transfer: Application to light-harvesting complexes. Appl Phys Lett 2007, 90:193901.
Wörmke S, Mackowski S, Brotosudarmo THP, Jung C, Zumbusch A, Ehrl M, Scheer H, Hofmann E, Hiller RG, Bräuchle C: Monitoring fluorescence of individual chromophores in peridinin-chlorophyll-protein complex using single molecule spectroscopy. Biochim Biophys Acta 2007, 1767:956–964.
Niklas J, Schulte T, Prakash S, van Gastel M, Hofmann E, Lubitz W: Spin-density distribution of the carotenoid triplet state in the peridinin-chlorophyll-protein antenna. A Q-band pulse electron-nuclear double resonance and density functional theory study. J Am Chem Soc 2007, 129:15442–15443.
Janus D, Hoff B, Hofmann E, Kück U: An efficient fungal RNA-silencing system using the DsRed reporter gene. Appl Environ Microbiol 2007, 73:962–970.
Hofmann E, Zerbe P, Schaller F: The crystal structure of Arabidopsis thaliana allene oxide cyclase: insights into the oxylipin cyclization reaction. Plant Cell 2006, 18:3201–3217.
Hermes S, Stachnik JM, Onidas D, Remy A, Hofmann E, Gerwert K: Proton uptake in the reaction center mutant L210DN from Rhodobacter sphaeroides via protonated water molecules. Biochemistry 2006, 45:13741–13749.
Gehlhar K, Rajashankar KR, Hofmann E, Betzel C, Weber W, Werner S, Bufe A: Lysine as a critical amino acid for IgE binding in Phl p 5b C terminus. Int Arch Allergy Immunol 2006, 140:285–294.
Brotosudarmo THP, Hofmann E, Hiller RG, Wörmke S, Mackowski S, Zumbusch A, Bräuchle C, Scheer H: Peridinin-chlorophyll-protein reconstituted with chlorophyll mixtures: preparation, bulk and single molecule spectroscopy. FEBS Lett 2006, 580:5257–5262.
Reinelt S, Hofmann E, Gerharz T, Bott M, Madden DR: The structure of the periplasmic ligand-binding domain of the sensor kinase CitA reveals the first extracellular PAS domain. J Biol Chem 2003, 278:39189–39196.
Steinke A, Grau S, Davidson A, Hofmann E, Ehrmann M: Characterization of transmembrane segments 3, 4, and 5 of MalF by mutational analysis. J Bacteriol 2001, 183:375–381.
Hiller RG, Crossley LG, Wrench PM, Santucci N, Hofmann E: The 15-kDa forms of the apo-peridinin-chlorophyll a protein (PCP) in dinoflagellates show high identity with the apo-32 kDa PCP forms, and have similar N-terminal leaders and gene arrangements. Mol Genet Genomics 2001, 266:254–259.
Kleima FJ, Wendling M, Hofmann E, Peterman EJ, van Grondelle R, van Amerongen H: Peridinin chlorophyll a protein: relating structure and steady-state spectroscopy. Biochemistry 2000, 39:5184–5195.
Kleima FJ, Hofmann E, Gobets B, van Stokkum IH, van Grondelle R, Diederichs K, van Amerongen H: Förster excitation energy transfer in peridinin-chlorophyll-a-protein. Biophys J 2000, 78:344–353.
Ferguson AD, Welte W, Hofmann E, Lindner B, Holst O, Coulton JW, Diederichs K: A conserved structural motif for lipopolysaccharide recognition by procaryotic and eucaryotic proteins. Structure 2000, 8:585–592.
Carbonera D, Giacometti G, Segre U, Hofmann E, Hiller R: Structure-based calculations of the optical spectra of the light-harvesting peridinin-chlorophyll-protein complexes from Amphidinium carterae and Heterocapsa pygmaea. J Phys Chem B 1999, 103:6349–6356.
Kleima F, Hofmann E, Gobets B, van Stokkum I, van Grondelle R, van Amerongen H: Peridinin chlorophyll protein: Structure and dynamics related. Photosynthesis: Mechanisms and Effects 1998, IV:441–444.
Ferguson AD, Hofmann E, Coulton JW, Diederichs K, Welte W: Siderophore-mediated iron transport: crystal structure of FhuA with bound lipopolysaccharide. Science 1998, 282:2215–20.
Ehrmann M, Ehrle R, Hofmann E, Boos W, Schlösser A: The ABC maltose transporter. Mol Microbiol 1998, 29:685–94.
Diederichs K, Hofmann E: Structural relation of peridinin chlorophyll A protein (PCP) and proteins with globin-like fold - A testable hypothesis for its folding and assembly pathway. New Methods for the Study of Biomolecular Complexes 1998, 510:277–284.
Hofmann E, Wrench PM, Sharples FP, Hiller RG, Welte W, Diederichs K: Structural basis of light harvesting by carotenoids: peridinin-chlorophyll-protein from Amphidinium carterae. Science 1996, 272:1788–1791.
Ehrle R, Pick C, Ulrich R, Hofmann E, Ehrmann M: Characterization of transmembrane domains 6, 7, and 8 of MalF by mutational analysis. J Bacteriol 1996, 178:2255–2262.

Citation Style: Current Opinion Journals